Banca de DEFESA: RODRIGO DA CRUZ FERREIRA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : RODRIGO DA CRUZ FERREIRA
DATA : 22/07/2021
HORA: 15:00
LOCAL: Sala da Plataforma Conferência Web (https://conferenciaweb.rnp.br/webconf/ppgbiotec-ufba)
TÍTULO:

Melipona quadrifasciata anthidioides, geoprópolis, multi-elements, IOES, HPLC.

PALAVRAS-CHAVES:

Melipona quadrifasciata anthidioides, geoprópolis, multi-elements, IOES, HPLC.

PÁGINAS: 247
GRANDE ÁREA: Outra
ÁREA: Multidisciplinar
RESUMO:

In order to characterize the gross geopropolis of Melipona quadrifasciata anthidioides, and to evaluate the physical stability of a semi-solid cream-type formulation incorporated into the assets of this geopropolis. Samples of this product were provided by the meliponary of the Rio Seco Agroecology Center (CEARIS), located in the city of Amélia Rodrigues-BA (UEFS), (latitude 12 ° 23'08 '' S / longitude 38 ° 47'51 '' W. The experiments were carried out in the laboratories: (LAEX-UEFS), (LABIV – UEFS), (IGEO UFBA), (LABMICRO-UFBA) (LAMABI / ICS / UFBA), (LAPEMEC) UFBA). Initially, a patent prospecting with the term "propolis" and "geopropolis" was carried out. Subsequently, the microbiota present in the geopropolis was verified in addition to the quantification of macro and microelements of the raw material through IOES. The extracts used were prepared using the Sohxlet technique using 5g of geopropolis diluted in 50ml with alcohol at 96 ° GL, then an analysis of the chemical profile was performed using the HPLC technique in addition to the evaluation of its antioxidant activities and antimicrobial against gram negative E.coli and Gram positive Staphylococcus aureus. The elaborated formulation was subjected to accelerated stability tests recommended by ANVISA. In vitro spread tests were also applied in order to outline the quality performance of a product with potential for topical application. Patent prospecting showed only 3 deposits with the term “geopropolis”. The results of the microbiota of the geoprópolis in natura demonstrated that the quantitative values of microorganisms indicating the microbiological criteria used for food were high for Bacillus cereus, aerobic mesophilic bacteria and molds and yeasts. With the absence of Salmonella sp. and Clostridium sulfite reducer. Macro and microelements (mg/ kg) were quantified (Al 320414,40-36911,1) (As <LD -4,37) (Ba 38,36-211,11) (Cd <LD) (Co <LD- 14,12) (Cr 17,41-38,07) (Cu 10,63-28,73) (Fe 21973,96- 11536,47) (Mn 50,51- 310,51) (Mo <LO) ( Ni 2,28-21,74) (Pb 3,45-8,55) (Sb <LD-1,64) (Se <LD-1,01) (Si <LD- 150,77) ( Sn 4,92-16,14) (Sr 9,21-36,29) (V 28,77-78,73) (Zn 24,34-50,31) (Ca 672,38-94527) (K 1974,38-9198,91) (Mg 1961,17-7481,79) (Na 154,55-340,46) (P 16,59-51,07) beyond C (2,26-7,69%). These extracts showed phenolic compounds and flavonoids in addition to exhibiting high antioxidant activity. The analysis of the chemical profile allowed to identify: gallic acid, naringenin, kampferol and kaempferide. The product formulated with (2.5%) showed the best stability under the stress conditions tested. Its ability to incorporate and accept the geopropolis ethanol extract was significant, keeping the characteristics of the stability tests unchanged.

MEMBROS DA BANCA:
Interno - 2765101 - ADEMIR EVANGELISTA DO VALE
Externo à Instituição - HUGO NEVES BRANDAO
Presidente - 118.484.305-82 - LIDERCIA CAVALCANTI RIBEIRO CERQUEIRA E SILVA - UFBA
Externa à Instituição - MARIA DA PUREZA SPINOLA MIRANDA
Externo ao Programa - 2476370 - MILLENO DANTAS MOTA